Recombinant Protein Expression

We offer a custom specific high-level recombinant protein expression services.

Bacteria

We offer a custom specific high-level recombinant protein E. coli expression service using a variety of commercially available strains. Expression optimization, flask and bioreactor production, rapid scale up, growth monitoring, protein refolding and tag removal.

  • Gene synthesis, codon optimization and cloning into suitable vectors
  • Expression screening to identify optimal conditions for expression
  • Scale of expression: 2 – 10 L lab scale and 20 – 225L pilot scale
  • Stability analysis of selected clones
  • Protein purification and characterization
  • Protein stability formulations

Yeasts

Yeast is very versatile in that combines both the advantages of prokaryotic and eukaryotic expression systems thus providing a system with growth speed and easy genetic manipulation with the cost-effectiveness of prokaryotic expression systems. PTL can offer:

  • A choice of Saccharomyces cerevisae and Pichia pastoris expression systems.
  • Proteins can be targeted for either intracellular expression or secretion into medium.
  • Protein purification and characterization.
  • Expression screening to identify suitable host strain and optimal conditions for expression
  • Scale of expression: 2 – 10 L lab scale and 20 – 225 L pilot scale
  • Protein stability formulations.

Microalgae

PTL offers a high-level algal expression service from research to industrial grade of recombinant proteins. This system also takes advantage of very low production costs. In addition PTL offer an innovative platform for the precise integration of DNA into predetermined sites within microalgal genomes.

  • Gene synthesis, codon optimization and cloning into suitable vectors.
  • Chlamydomonas reinhardtii
  • Confirmation of protein expression by initial transient transfection trials
  • Transfection and stable pool generation using suitable selection and amplification
  • Clonal selection by limiting dilution and screening of clones
  • Productivity and stability analysis of selected clones.
  • Protein purification and characterization
  • Protein stability formulation.

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Plants

PTL can offer Agrobacterium infiltration-mediated transient and stable transformation for a rapid and highly efficient protein expression in plants. Using tobacco plants, secretion, post-translational modifications and high expression of recombinant proteins ranging from recombinant enzymes to therapeutic targets and vaccines can be achieved.

  • Gene synthesis, codon optimization and cloning into suitable vectors.
  • Confirmation of protein expression by initial transient transformation trials.
  • Transfection and stable pool generation using suitable selection and amplification.
  • Clonal selection by limiting dilution and screening of clones.
  • Productivity and stability analysis of selected clones.
  • Protein purification and characterization.
  • Protein stability formulations.

Insects

Baculovirus is a powerful and versatile system for producing high levels of recombinant protein expression in insect cells. The advantages of insect cell expression are that it offers post-translational modifications similar to mammalian cells with high levels of protein expression and ease of scale-up.

We offer:

  • Insect cell expression using baculovirus or S2 drosophila cells.
    • Baculovirus cell expression using protease deficient baculovirus DNA.
    • A choice of cell lines: Sf9, Sf21 or Hi5.
    • Generation of high titer recombinant baculovirus.
  • MOI studies.
  • Proteins can be targeted for either intracellular or secretion.
  • Protein purification and characterization.
  • Protein stability formulations.
  • Scale of expression: lab-scale to 50L in Wave Bioreactor