Recombinant Protein Expression

We offer a custom specific high-level recombinant protein expression services.

Bacteria

We offer a custom specific high-level recombinant protein E. coli expression service using a variety of commercially available strains. Expression optimization, flask and bioreactor production, rapid scale up, growth monitoring, protein refolding and tag removal.

  • Gene synthesis, codon optimization and cloning into suitable vectors
  • Expression screening to identify optimal conditions for expression
  • Scale of expression: 2 – 5L lab scale
  • Stability analysis of selected clones
  • Protein purification and characterization
  • Protein stability formulations

Yeasts

Yeast is very versatile in that combines both the advantages of prokaryotic and eukaryotic expression systems thus providing a system with growth speed and easy genetic manipulation with the cost-effectiveness of prokaryotic expression systems. PTL can offer:

  • A choice of Saccharomyces cerevisae and Pichia pastoris expression systems.
  • Proteins can be targeted for either intracellular expression or secretion into medium.
  • Protein purification and characterization.
  • Expression screening to identify suitable host strain and optimal conditions for expression
  • Scale of expression: 2 – 5L lab scale
  • Protein stability formulations.

Plants

PTL can offer Agrobacterium infiltration-mediated transient and stable transformation for a rapid and highly efficient protein expression in plants. Using tobacco plants, secretion, post-translational modifications and high expression of recombinant proteins ranging from recombinant enzymes to therapeutic targets and vaccines can be achieved.

  • Gene synthesis, codon optimization and cloning into suitable vectors.
  • Confirmation of protein expression by initial transient transformation trials.
  • Stable transformation and selection of regenerants.
  • Productivity and stability analysis of selected transformants.
  • Protein purification and characterization.
  • Protein stability formulations.

Microalgae

PTL offers a service for expression in Chlamydomonas reinhardtii of recombinant proteins. This system also takes advantage of very low production costs. In addition PTL offer an innovative platform for the precise integration of DNA into predetermined sites within microalgal genomes.

  • Gene synthesis, codon optimization and cloning into suitable vectors.
  • Transformation trials and selection of transgenic algal clones.
  • Confirmation of protein expression.
  • Productivity and stability analysis of selected clones.
  • Protein purification and characterization.
  • Protein stability formulation.